红树植物—白骨壤黑斑病病原菌的分离与鉴定

<正>红树林是生长在热带、亚热带海岸潮间带,可海陆两栖的特殊木本植物群落,具有防风御浪、净化环境、维护沿海湿地生物多样性等生态功能,为珍稀植物群落。有报道认为红树植物生长在海边又富含抗菌等活性物质,不利于病害发生,但近年来本实验室在广东雷州半岛等地红树林发现有多种病害发生~([1,2]),仅白骨壤(Avicennia marina)上就发现有黑茎病、灰斑病等病害。2014年我们在该区白骨壤小苗     

Assessment of molecular detection of Fusarium circinatum in insects and passive spore traps in Pinus radiata plantations

Fusarium circinatum is the causal agent of pitch canker, a destructive disease that threatens natural and planted pine forests around the world. Although pitch canker has caused problems in Spain and Portugal, concerning Europe as a whole, the fungus is not established across the pine distribution area. Its dispersion by wind and/or insect vectors could nevertheless play a role in the colonization of currently uninfected stands. It is therefore crucial to develop monitoring tools for its detection. To this end, we assessed the molecular detection of the pathogen in environmental samples of bark beetles and passive spore traps, collected in two infected Pinus radiata plantations in Basque country, Spain. The spread pattern of F. circinatum was assessed by an experimental design that included insect and spore traps installed at the centre, at the edge and outside the plots. Our results showed that F. circinatum was detected in both types of samples, at almost all collection dates. In both type of samples, positive detections were mainly found at the centre of the plots, a lower proportion at the edge, and very few outside. This suggests that long‐distance dispersion of Fusarium circinatum does not rely on wind spore dispersal neither on insect flight. Our study also shows that molecular methods are a powerful tool to monitor the pathogen in environmental samples.     

茶树小分子量热激蛋白基因CsHSP17.2的克隆与表达分析

[目的]进一步了解茶树小分子量热激蛋白基因CsHSP17.2在逆境胁迫条件下的分子生物学功能.[方法]利用RT-PCR技术从茶树‘迎霜’中克隆得到CsHSP1 7.2基因,运用生物信息学软件分析其核苷酸和编码蛋白,通过Real-timePCR分析其表达模式.[结果]该基因开放阅读框长度为453 bp,编码150个氨基酸,蛋白质相对分子质量为17.2×10a,理论等电点5.56;无信号肽位点,属于非分泌型蛋白;被定位于细胞质中.系统发育树分析表明,茶树CsHSP1 7.2与水稻(GenBank登录号:P27777)和花生(ABC41131)的进化关系较近,属于小分子量热激蛋白基因家族第Ⅰ亚族.qRT-PCR分析发现,茶树CsHSP17.2属于组成型基因;高温(38℃)处理1h能显著提高CsHSP17.2 mRNA的相对表达量(P＜0.05);干旱(100 g·L-1 PEG 6000)、高盐(200 mmol· L-1 NaCl)和外源脱落酸(200 mg· L-1 ABA)处理条件下,该基因的转录水平均出现不同程度的上调.[结论]克隆得到茶树‘迎霜’小分子量热激蛋白基因CsHSP17.2,其在花中表达量最高,且响应高温、干旱、高盐和外源脱落酸胁迫.     

动态细分饲粮营养供给对肉鸡生产性能、肉品质、氮与磷代谢率及血清生化指标的影响

本试验旨在根据肉鸡生长曲线及动态营养需要,研究动态细分饲粮营养供给对肉鸡生产性能、肉品质、氮、磷代谢率及血清生化指标的影响。选取1日龄爱拔益加(AA)肉鸡240只(公母各占1/2),随机分为3个组,分别为A组:每3.5 d 1个营养标准(1~7日龄1个营养标准);B组:每7 d 1个营养标准;C组:每14 d 1个营养标准。每组8个重复,每个重复10只鸡,试验期42 d。结果表明:1)C组肉鸡的平均日增重(ADG)显著高于A、B组(P0.05),料重比(F/G)显著低于A组(P0.05)。A、B组肉鸡生产性能指标差异均不显著(P0.05)。不同动态细分饲粮营养供给方式对肉鸡肉品质的影响无显著差异(P0.05)。2)不同动态细分饲粮营养供给方式对肉鸡氮、磷代谢率的影响无显著差异(P0.05)。3)肉鸡28日龄时,C组肉鸡血清尿素氮(UREA)含量显著高于A组(P0.05);42日龄时,C组肉鸡血清UREA含量显著高于A、B组(P0.05),但A、B组肉鸡血清UREA含量差异不显著(P0.05)。4)不同动态细分饲粮营养供给方式对肉鸡血清皮质酮(CORT)含量及总抗氧化能力(T-AOC)的影响差异不显著(P0.05)。综上所述,每14 d 1个营养标准可使肉鸡的生产性能达到最佳,从实际操作的复杂性考虑,每7 d 1个营养标准可更好地满足饲粮的氨基酸平衡。     

GDFs基因家族候选基因对崇仁麻鸡生长性状的影响

利用崇仁麻鸡差异性状的重测序结果,将生长分化因子(GDFs)基因家族作为候选基因,筛选与崇仁麻鸡生长性状相关的多态位点。结果发现,GDF-2、GDF-8和GDF-10基因的多态位点对崇仁麻鸡的生长性状有着显著或极显著的影响。单倍型分析结果显示,GDF-2和GDF-10基因的单倍型H3H3为劣势单倍型组合可以选择淘汰。研究结果表明,GDFs基因家族候选基因对崇仁麻鸡的生长性状有一定的影响。     

黔南州核桃优树叶片营养元素和果实品质相关分析

为了分析叶片营养元素对黔南州核桃优树坚果品质的影响,通过方差分析和相关分析研究了叶片营养元素与坚果品质之间的相关关系。结果表明:不同品种的核桃优树单株的坚果品质指标间存在显著差异;叶片钙、铜和锰显著影响核桃坚果品质。可以通过核桃叶片营养元素的诊断来提高坚果品质。     

论如何有效实施营养宣教

营养宣教对于慢性病的预防、保障大众健康、降低医疗支出具有重要意义。从多个角度论述如何有效进行营养宣教，列举一些可行措施，以期使营养宣教工作的实施有所借鉴。     

Identification and evaluation of high nitrogen nutrition efficiency in rapeseed (Brassica napus L.) germplasm

To establish identification and evaluation methods of N (nitrogen) absorption and utilization of rapeseed (Brassica napus L.), difference of N nutrition efficiency (NNE) among rapeseed germplasms and relationship between NNE and plant traits under various N application rates were analyzed in this research. Pot cultivating experiments were conducted to investigate NNE with 3 ​N application rates in soil (0.05, 0.2 and 0.3 ​g/kg). A total of 12 rapeseed germplasms were planted, nitrogen absorption efficiency (NAE) and nitrogen utilization efficiency (NUE) in seedling stage, bolting stage, initial flowering stage, final flowering stage, and maturity stage were obtained. Results showed that bolting stage was the best period for NAE identification and evaluation. Low N application rate in soil (0.05 ​g/kg) was the best for NAE, and the indirect indexes were basal stem diameter, plant root dry weight and above ground plant dry weight. Maturity stage was the best period for NUE identification and evaluation. High N application rate in soil (0.3 ​g/kg) was the best for NUE, and indirect indexes were above ground plant dry weight and basal stem diameter. N application rates of 0.05 ​g/kg in soil was the best for nitrogen harvest index at maturity stage, and indirect indexes was number of pods per plant. Plant traits of rapeseed germplasms affected NNE. Higher basal stem diameter, plant root dry weight and above ground plant dry weight at bolting stage under low N application rate were important characteristics of N absorption in rapeseed. Higher above ground plant dry weight and basal stem diameter at maturity stage under high N application rate were important characteristics of N utilization. Higher number of pods per plant at maturity stage under lowe N application rate was an important characteristic of N harvest index. These results provided a reliable index for N management and provided theoretical basis for guiding rapeseed breeding.     

An efficient method to produce segregating populations in quinoa (Chenopodium quinoa)

Quinoa offers a promising alternative for staple food, considering its outstanding nutritional value and tolerance to abiotic stresses. To develop breeding programmes in quinoa, a reliable crossing method for increasing the genetic variation is required. In the following study, we aimed to develop segregating populations in quinoa. We tested the efficiency of three different crossing methods (hand emasculation, warm water emasculation and no emasculation). Moreover we developed a two-stage selection strategy based on morphological traits and molecular markers for the selection of hybrid plants. We reported hand emasculation to be the most efficient crossing method, followed by warm water emasculation and no emasculation. Our results demonstrated that crosses in quinoa can be successfully performed, despite its complicated flower structure and high self-pollination rate. Additionally, we developed 30 segregating populations from crosses between accessions of different origins with varying phylogenetic relationship, which offers a promising perspective for quinoa breeding programmes in the future.     

鸡无尾性状的分子机制研究进展

动物的尾巴具有改变方向、控制升降、调整速度、支撑身体、防御、攻击、保温、示警、逃生和捕食等作用。鸟类的尾巴在飞行过程中起到平衡身体的关键作用，但在进化过程中尾部却出现了缩短和融合现象，如中国的瓢鸡及智利的Araucana鸡。作者简述了近年来关于鸡无尾性状的解剖学研究成果，发现无尾鸡在胚胎发育HH19期（Hamburger和Humilton标准分期）就已经出现无尾现象，而在结构上无尾鸡缺乏尾脂腺、尾羽、镰羽、尾椎骨和尾综骨。同时作者分析了导致尾部体节停止发育的分子遗传机制，包括周期性表达基因的转录调控影响尾部延伸过程、后端化因子梯度诱导尾部形成、Hox基因影响体节特化过程及基因突变有可能导致的无尾现象等，从而提出了可能导致家禽无尾性状的胚胎发育时期的关键分子及信号通路，包括Irx1和Irx2基因、Notch信号通路、Wnt信号通路、Fgf信号通路、RA信号通路等。研究鸡无尾性状的分子机制不仅可深入了解脊椎动物胚胎发育中尾部发育机制，更有利于揭示鸟类在进化过程中发生的尾部缩短及融合的机理。